Fig. 3

Blockade of TGFβ or IL-10 signaling does not reverse vitamin D mediated IL-13 cytokine production. CD11c + DCs with from patients with confirmed ABPA before (D0) and after supplementation with 4000 IU cholecalciferol daily were treated with a and c) media or b and d) TSLP (5 ng/ml) and then pulsed with Aspergillus Extract followed by addition of allogeneic bulk CD4+ T-cells followed by addition of a and b) sTGF-βII/FC (10 μg/mL) or c and d) anti-IL-10 (1 μg/mL). Cells were incubated for 96 h and IL-13 was measured in cell supernatants by Luminex. Data is graphed as a percentage of the stimulated response (CD3/CD28) for each individual patient at each time point